奶牛乳腺上皮细胞中SCAP和SREBP1蛋白调控SCD基因表达的作用研究

doi:10.11843/j.issn.0366-6964.2018.03.024

Abstract

Abstract:

The aim of this study was to investigate the effect of sterol regulatory element-binding protein cleavage activating protein (SCAP) on the expression of stearoyl-CoA desaturase(SCD) gene regulated by steroid regulatory element binding protein (SREBP1) in dairy mammary epithelial cells. The dairy mammary epithelial cells were cultured and transfected with SCD gene promoter vectors, then SREBP1 and SCAP eukaryotic expression vectors were transfected into cells as treatment factor.The SCD promoter activity was detected by dual luciferase reporter gene system.The expression of SREBP1 in nucleus was observed by immunofluorescence. qRT-PCR was used to measure the mRNA expression of SCD gene. The results showed that, compared with pcDNA3.1 control group, transfection of SCAP had no significant effect on SCD promoter activity. Transfection of SREBP1 and co-transfection of SCAP/SREBP1 significantly increased the SCD promoter activity (P<0.01). There was a significant dose-response relationship between the transfection dose of SCAP and the promoter activity of SCD (P<0.01). The expression of SREBP1 in nucleus could be enhanced by SCAP transfection in mammary epithelial cells. The mRNA expression of SCD was up-regulated by 1.23-fold and 1.54-fold (P <0.05) after transfected SREBP1 or co-transfected SCAP/SREBP1, respectively. In conclusion, SCAP can increase the expression of SREBP1 protein in nucleus which enhance the transcriptional activation of SCD gene.

CLC Number:

S823.2

HAN Li-qiang, SUN Yu, FU Tong, LIAN Hong-xia, GAO Teng-yun. Effect of SCAP and SREBP1 Proteins on Regulation of SCD Gene Expression in Dairy Mammary Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 652-658.

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Effect of SCAP and SREBP1 Proteins on Regulation of SCD Gene Expression in Dairy Mammary Epithelial Cells

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